SGA preprocess error, read ids

HerefordGuy

Junior Member

Join Date: Apr 2010

Posts: 6
Share

Post
#1

SGA preprocess error, read ids

06-11-2011, 07:36 AM

SGA preprocess is giving me the following error:

Warning: Pair IDs do not match (expected format /1,/2 or /A,/B)
Read1 ID: HWUSI-EAS558R_0015:7:26:4067:7956#0/1
Read2 ID: HWUSI-EAS558R_0015:7:26:4067:7956#0/2

When I forgot and left the --phred64 tag off, it thought the ends of the pair ids were part of the quality scores.
My question, are the read ids too long for SGA? If so how would I change this? Or is this a problem with Bamtools, which SGA uses?

Thanks!
Jared
Tags: bamtools, paired ends, read id, sga

Previous template Next

Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool

by SEQadmin2

CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.¹ But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

Despite this, “CRISPR helped turn genome editing from a specialized technique into...
- Channel: Articles
07-31-2026, 11:01 AM
Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications

by SEQadmin2

Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

The systematic characterization of the human proteome has...
- Channel: Articles
07-20-2026, 11:48 AM
Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems

by SEQadmin2

Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...
- Channel: Articles
07-09-2026, 11:10 AM

Topics	Statistics	Last Post
Consortium Completes First Full Diploid Human Genome by SEQadmin2 Started by SEQadmin2, Today, 07:41 AM	0 responses 9 views 0 reactions	Last Post by SEQadmin2 Today, 07:41 AM
Long-Read Sequencing Closes Decades-Old Gaps in the Medaka Genome by SEQadmin2 Started by SEQadmin2, 08-03-2026, 10:13 AM	0 responses 23 views 0 reactions	Last Post by SEQadmin2 08-03-2026, 10:13 AM
New Genomic Method Uncovers Ancient Hominin DNA by SEQadmin2 Started by SEQadmin2, 07-31-2026, 02:55 AM	0 responses 38 views 0 reactions	Last Post by SEQadmin2 07-31-2026, 02:55 AM
Study Captures the First Moments of DNA Replication by SEQadmin2 Started by SEQadmin2, 07-24-2026, 12:17 PM	0 responses 25 views 0 reactions	Last Post by SEQadmin2 07-24-2026, 12:17 PM

Unconfigured Ad

SGA preprocess error, read ids

Latest Articles

ad_right_rmr

News