8 results in 0.0030 seconds.
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STACKS and PE Illumina Data (ddRAD)
I have a dataset from a ddRAD library (Illumina HiSeq4K, 150PE). I'm playing around with STACKS, and noticed that it has some limitations when dealing... -
To remove or to keep duplicates in alignment of NGS paired reads to a set of contigs
I have a SAM file containing alignments of NGS paired reads against a set of assembled contigs (de novo).
If I might need to infer some... -
454 ACE assembly with paired reads... how to view?
Hi,
I'm new here, thanks in advance for any help.
I have a 454 dataset of 'allcontigs.ace'. The reads consist of paired and... -
Samtools: Paired reads with no Ref. name of the mate
Hi,
In some bam files from paired reads data I see some of them with the field RNEXT or reference of the mate equal to "*". In these... -
SAM flag forward/reverse first/second
Hello,
I am a little confused with the interpretation of SAM flags
From http://ppotato.wordpress.com/2010/08...wise-flag-pair... -
Bowtie 2 parameters
Hi All,
I am using bowtie 2 to align some illumina sample data on an influenza genome which has less than 14k nt. The samples have more... -
pair reads vs single reads
Hi,
I am very new to deep sequencing. I have a small project using RNA seq data
SRA010053 (mouse fibroblasts), SRA040235 (mouse ES cells).... -
Unpaired reads in paired data
I have a paired read dataset in sam format. However some of the reads are unpaired with #0 a the end of their names. The flags indicate these are all...
