Hi,
In some bam files from paired reads data I see some of them with the field RNEXT or reference of the mate equal to "*". In these cases which flags should or not be used? Some examples of these reads are:
Thanks
In some bam files from paired reads data I see some of them with the field RNEXT or reference of the mate equal to "*". In these cases which flags should or not be used? Some examples of these reads are:
Code:
WESLEY:8:80:156:228/1/2 129 chr1 566704 255 69M * 0 0 CCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTACCCC SWTTZ^WWYQYLQTQU^RRWZ`_ZROQ_^YYW^_aa_aa_`a^aaabaaababaaba`^``baa_bbbb SOCAC:1:25:373:836/2/1 65 chr1 566430 255 4S54M18S * 0 0 CTGTAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCGTTGACTAATCCCTACAGATCGGAAGAGCGTCGCG abbabbbbbaaabbbababbaaabbaaZH[^]ab\aa]^aa\S_aaa\`Za`]_a`a^Ua_]LYL\MVXaYU^BBB SOCAC:2:75:1643:1924/2/2 145 chr1 566428 255 63M6S * 0 0 TCAGCCATTTTACCTCACCCCCACTGNTGTTCGCCGACCGTTGACTATTCTCCACAAACCACAGATCGG a`bbaabababb`^baba`Wa`a[`^D\baa`\L\a_aZa_[``_`]a```]KYY]``WBBBBBBBBBB