hello, everyone! I am a new member here.
Recently, I am working with the bacterial RNA-SEQ data. My data was in strand-specific PE reads fq file....
5 results in 0.0028 seconds.
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how to caculate rpkm for bacteria RNA-SEQ data?
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Filter PE reads together
Hi,
I'm working with RNA-Seq using 65 bp paired end reads. I'm having huge problems with mapping - after trimming and filtering reads (using... -
Using quality filtered fastq files with TopHat
Hi all,
I want to use tophat with my paired-ends RNA-Seq data, which are in fastq format. The problem however is that after I quality filter my... -
Library preparation
Hello everybody,
I would like to know what is the difference between the mate-pair and paired-end preparation for NGS and how these results... -
Error during PicardMarkDuplicates (Illumina paired-ends mapped by Bfast)
I mapped one run of Illumina paired end reads by using Bfast (bwaaln + localalign + postprocess), then converted it to bam, performed the Picard AddOrReplaceReadGroups...
