Hi,
I've been looking for the PCR regions or 'manifest file' for a whole genome sequencing kit? In an example there hundreds of WGS in...
9 results in 0.0055 seconds.
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Manifest File for Whole Genome Sequencing
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why Hairspin region will affect the PCR product
Hi,
I am doing a primer design recently, and using the primer 3 to do the primer design. But get a warning at the end:
CGGCGCCGAGCCCCGCCGGGGTGAGCTGGG... -
Nextera indexing and taq polymerases
Hello!
I am trying to sequence (Miseq) a custom amplicon from many samples using the Nextera indexes. For those of you not familiar, this... -
16S V4 Indexing PCR - Thoughts on this gel?
Hi all
I'm running a microbiome analysis of fish gut microdlora using Illumina MiSeq 16s pipeline. I'd like to get your thoughts on my... -
Variant found in priming site
I sequenced barcoded PCR amplicons on Ion Torrent PGM, and a subset of samples were also Sanger sequenced to confirm variable sites.
I... -
Extra peaks in a bioanalyzer trace for library made from ePCR amplicon
Hello,
I am working on a deep sequencing protocol for a PCR amplicon (~650bp) using the TruSeq DNA PCR-Free Sample Preparation Kit and... -
RNA-seq: concern about quantification of transcriptome
Hi everybody!
I'd like to generate and quantify whole transcriptomes by using RNA-seq in the near -- well, not so near -- future but I... -
PacBio RS II Library Prep Input
Hi all,
I'm looking to sequence low quantities of PCR amplicon using the PacBio RS II system. I am trying to detect single nucleotide variants... -
ISSR amplicon sequencing on Illumina
Hi all,
I'm new to SEQanswers and a PhD student at Ohio State. A faculty member recently purchased a PCR amplicon sequencing kit from Illumina...
