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PCR bias in library construction
I am thinking of investigating PCR bias in NGS library preps. I have seen a review and a few articles detailing bias with different polymerases. Kappa... -
Debugging PCR-based amplification of mutant library
Dear all,
I've come across a rather odd case of PCR problems with a mutant library, which is used in directed evolution experiments. The library... -
Lib prep - Splitting PCR to reduce PCR bias
I want to reduce my high levels of duplicate reads (>50%) in my illumina HiSeq 100bp Paired end sequences.
The normal solutions aren't possible...
