Dear all,
im performing a protocol in which i circularize the cDNA and then do the amplification PCR of the libraries.
I process many samples,...
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Varying n of PCR cycles and biases in quantitative analysis
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Debugging PCR-based amplification of mutant library
Dear all,
I've come across a rather odd case of PCR problems with a mutant library, which is used in directed evolution experiments. The library... -
dulpicate reads from ampicon/PCR based library
I am having trouble getting my head round the issue of duplicate reads and in particular how this works with data derived from a amplicon generated PCR...Last edited by yog77; 07-30-2012, 03:28 AM. -
Fluidigm PCR amplicon Library prep
Hi has any one got any advice on a two-step PCR amplicon library generation via barcode/adapter addition using a secondary PCR.
I am... -
Sequencing advice
I am planning to sequence 60kb gene using ovelapping long range PCR, I have designed primers and amplified whole gene. I am running into problem now because...
