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Doing PCR enrichment on PCR-free TruSeq lib?
I am making a contingency plan in case our next TruSeq (DNA) PCR-free libraries don't have sufficient yield. As far as I can tell the only difference... -
inefficient NEB DNA library prep with plenty of starting material
Here's the short description:
We want to ligate adaptors and indexes to 1-3μg of sheared DNA and, in the end, have at least 500ng of adaptor-ligated... -
PCR-free; absolute abundance; yeast
I am new to NGS.
Goal is to find absolute abundance of yeast species in a mixed sample.
I plan to use PCR-free methods since PCR could cause... -
Extra peaks in a bioanalyzer trace for library made from ePCR amplicon
Hello,
I am working on a deep sequencing protocol for a PCR amplicon (~650bp) using the TruSeq DNA PCR-Free Sample Preparation Kit and... -
TruSeq PCR-free transition
Hi together,
my lab is planning to switch to the TruSeq PCR free library prep. We would like to perform size selection on a bluepippin. Reading...Last edited by avo; 11-15-2013, 04:13 AM. -
PCR-Free Libraries & Bioinformatic PCR Duplicate detection?
Does any one have any thoughts or experience with regards to the Bioinformatics pipeline for Illumina PCR-Free libraries?
Especially with...
