Dear community,
I need some help, as RNA-sequencing is quite new for me...
I prepared DNA libraries from RNA for sequencing with illumina....
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High peak at 240nm in libraries - does it matter?
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TruSeq DNA PCR-free Sample Preparation Kit (Part#15036187 Rev.A):extra peak, low qPCR
Subject: TruSeq DNA PCR-free Sample Preparation Kit (Part#15036187 Rev.A): validate library showed extra peak by Bioanalyzer and low qPCR concentration...
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The distribution of peak in ChIP-seq on function element
I have got some peaks from a ChIP-seq data.
Now I'm trying to locate these peaks on the function elements.
But I come up with a question...
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Inconsistency between biological replicates
Hello,
So i am relatively new to the whole chip-seq analysis.
I have 2 different biological replicates for the same protein...
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Can I randomly sampe from ChIP seq input
I have a problem for peak calling. Could anyone help me?
After bowtie, I am using MACS to call peaks. There are 12 million unique reads in input,...
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Software for determining Genes near peaks?
Hi all,
I am interested in getting the list of genes near to my peaks. I have tried different packages but they only give you the nearest...
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Method paper: Model-based Analysis of ChIP-Seq (MACS)
Saw this paper on apfejes' blog, thought it was pretty interesting as a second generation of peak finders.
http://genomebiology.com/2008...