Hello Everyone!
I'm Zimtblatt and I am happy to have found this community.
I'm from a biology background and have been analyzing...
16 results in 0.0031 seconds.
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New to 16S Seq on MiSeq
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Too little PhiX - problem with denaturation?
Hi all,
first the TLDR:
Do you know of any factors that might cause PhiX denaturation to fail or reverse, outside of typical mistakes... -
Reduce PhiX
Dear all,
I was wondering if there was any tool to help me reduce the PhiX% used in our NextSeq 500 runs? We run similar samples continuously,... -
Experienced sequencing providers for RRBS-seq?
Hi,
can someone recommend me a reliable sequencing provider for RRBS-sequencing? Our current provider struggles with this kind of application,... -
How much phiX to spike in for ddRADseq library on HiSeq?
Our lab is preparing for a large scale ddRADseq project (~3000 samples). In doing the calculations for how many lanes of sequencing we will need to achieve... -
PhiX sequences in Miseq Output
I did a run using miseq (v3 chemistry, paired end 2x300, 25% PhiX added). I did a own designed pcr based library preperation.
Now for... -
phiX on Bioanalyzer
In case anyone else was curious. This is Illumina's ΦX174 reference library ("PhiX Sequencing Control V3", p/n FC-110-3001, allegedly 10... -
Why and how: PhiX SPike-in?
Hi Seqanswer team, and forum members,
Pardon me if this seems to be a broad and naive question.
I do not use sequencing kits,... -
16S Miseq run with 96 indexed samples
Hi everyone,
First post here, sorry for the bad writing.
Me and my colleagues are planning to do a Miseq run to examine the 16S RNA of several... -
Sequence of PhiX MiSeq primers
Newbie trying to track down source of failed (~10% of clusters pass filter) MiSeq amplicon sequencing run. I want to look at possible interactions between... -
Custom primers and PhiX on MiSeq
Hi all,
I have a question about using custom primers to sequence 16S rRNA using MiSeq. I have not done a run since last April and while reviewing... -
Genomic version of PhiX?
Basically my lab's looking for a version Phix that has not been sheered, doesn't have any adaptors or barcodes on it, etc., etc. to use as a in-line control... -
Cluster density on a HiSeq2500
We are in the midst of our HiSeq2500 upgrade. First one for our intrepid FSE. Still some kinks in the process -- like one of the rear manifolds had an... -
How much PhiX in MiSeq run?
Hello all -
Our lab is about to run a RADseq library on an Illumina MiSeq. It consists of multiplexed individuals with semi-random fragments... -
Need Phix control sequence.txt
We just lost all our raw and archived Solexa data in a stupid SAN controller crash.
Not a scientific disaster, but I have a student...
