Hi,
I recently used the MiSeq for a 2 x 76 paired-end sequencing run.
I moved the 'L001' directory from BaseSpace to our...
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Picard tools error
Hi,
I am attempting to use CollectRnaSeqMetrics from picard tools on my BAM files but I am not being successful with it for some reason....Last edited by ea11; 01-08-2016, 01:02 AM.
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Picard CalculateHsMetrics Function - what do all the output abbreviations mean?
Hi,
I am using the CalculateHsMetrics in Picard to estimate the per cent of on and off target reads for some next generation sequence data...
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Exclude certain tags from BAM file
Wishing you guys have a nice Mayday.
I want to exclude certain reference tags(genes) from BAM files and direct the output to a new BAM...
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Picard Tools "loaded 0 genes"
I am testing the CollectRnaSeqMetrics script in Picard Tools for the first time and received the following error message:
INFO...
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GATK RealignerTargetCreator Error = Contigs do not match
I'm trying to do a local realignment using GATK and seem to be stuck at the first step. I've created my ref.dict file and then tried the following script:...
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Help in converting Sam to Bam!
Hello again everyone...
So, today I was happy as I got all my fastq files aligned and thought today's a beautiful day and all will go when...
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What exactly is AddOrReplaceReadGroups (picard tools) doing?
Hello folks,
I struggled now a few days, but I don't get it:
What exactly is AddOrReplaceReadGroups doing?
http://pic...