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Announcing the Genetic Engineering Attribution Challenge!
altLabs is excited to announce the launch of our new data science competition Genetic Engineering Attribution Challenge! We’re awarding $60,000...
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Question on multiplexing - long reads
Hi All
So I have 2-3 number of 2MB bacterial genome and two plasmids of 17 kb each length. I am looking at the feasilbility of multiplexing...
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Sanger sequencing
Can anyone help me in understanding how plasmids are sequenced using the chain termination method?
Thanks.
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Plasmid design using APE
Hey guys, I'm a newbie to bioinformatics and still doing an undergrad. But I really needed help with something and hope you guys can. I am designing an...
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Cluster complete plasmid sequences
Hey all,
I have complete nucleotide sequences for 119 plasmids, with sizes ranging from 500 to 170000 base pairs. Is there a way to cluster those...
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Building a Circular de novo Assembler
Geneious developer Matt Kearse has written a blog about how he built the circular de novo assembler for the R7 Update. Hopefully some of you will find...
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Filtration of plasmid metagenome paired end reads
Hi all,
I am studying plasmids metagenome from clinical samples. The plasmids were captured from metagenomic DNA by digestion of linear...
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:confused: Integrated plasmid
Hi all,
We think that a genome we sequenced recently has a integrated plasmid. We have found plasmid related proteins within the annotation,...
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extracted sequences from sff
Hi,
Has anyone successfully extracted a subset of sequences by their read ID from an sff file? I'm trying to separate plasmid and chromosomal...