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JoeTaylor started a topic RNAseq library for polysome profiling (monosome and polysome-associated RNA)

in Sample Prep / Library Generation

11-19-2020, 12:08 PM
RNAseq library for polysome profiling (monosome and polysome-associated RNA)

Hi there,

I am interested in performing polysome profiling with downstream application for RNAseq of monosome and polysome-associated RNAs....

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nboom started a topic high rRNA after poly(A) selection

in Sample Prep / Library Generation

10-23-2019, 11:59 PM
high rRNA after poly(A) selection

Hi,

About half our samples post library prep (poly(A) selection) + sequencing had around 50% rRNA content. The samples were re-prepped...

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SS00 started a topic Why can't I just add Poly A to my Total RNA?

in Sample Prep / Library Generation

11-29-2013, 03:06 AM
Why can't I just add Poly A to my Total RNA?

Hi all,

I have been weighing up my options with low input RNA. A bit of a debate has been going on regarding Poly A vs Total RNA in my...

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