Hi everyone,
I performed mapping and differential expression analysis of PolyA selected RNASeq libraries and now I'm going to get more...
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Combining Ribozero and PolyA data
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PolyA removal from RNA seq Illumina reads
Hi All,
I have paired end RNA seq Illumina reads. Do i need to remove the PolyA from the reads ( is it required) in the preprocessing step...
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% rRNA remaining after polyA selection
Does anyone have a standard or threshold of % rRNA they require in order to proceed with mRNA library prep?
In the past I've only proceeded...
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Polyadenylation site detection
Hi,
I assembled transcripts using TopHat/Cufflinks pipeline using Illumina paired-end RNA-Seq reads. I am working with human transcriptome data....
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PolyA reads are disappeared?
Hi at all,
I've done an RNA-seq experiments with SOLiD 4 using a polyA-enriched RNA as the input sample. Interestingly I've found only few reads...