Hi guys,
a quick question. I am new to pacbio. I've just received pacbio data for assembling a genome (low coverage). It comes from a highly...
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Pacbio assembly of polymorphic genome
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Samtools is failing to phase bam files accurately
My colleagues and I are assembling transcriptomes from RNASeq data. Our pipeline can (loosely) be outlined as follows:
1. De novo assembly using... -
mutation detection but has to exclude known polymorphism?
Recently got a project on human data. It is easy to find out all the mutations. But how do I exclude all the know Polymorphisms, any approach? Thanks... -
Virus polymorphism Segminator
I have been using GATK tools (mapping done with Mosaik) and Segminator II (using FASTQ data) to map polymorhism in virus resequencing (Ion Torrent). Comparing... -
Polymorphism in de novo assembled transcriptomes
Hi, i am a post-doc who recently turned to bioinofrmatics. My institute has a nice facility, but my lab is very inexperienced so I could use some external... -
impact of polymorphism on assembly
Hi everyone,
Has anyone assessed the influence of polymorphism (within species) on transcriptome assembly? I have not stumbled upon anything...
