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Multi-marker metabarcoding: how much length difference will give marker bias?
Hello SEQanswers community! I want to sequence bacterial, fungal, plant and arthropod libraries on one MiSeq run from the same faecal samples. I know... -
ATAC-seq library sequencing
Hi,
I am about to submit my first mouse ATAC-seq libraries for sequencing, but I have doubts regarding the number of libraries to pool... -
Pooling samples from different genomes
I would like to sequence genomes from 2 different organisms using Illumina Miseq. one is Staph. aureus of about 2.8Mbp and the other is a virus of about... -
pooling libraries across multiple lanes
Dear SEQanswers experts - I have a set of 24 samples for RNAseq. The analyses I need to do is pairwise (i.e. 12 pairs of samples) for differential gene... -
Pooling reads from different libraries
Hi all,
I have a library I have been given to work with but am having trouble working out if my approach is legitimate. I have 3 libraries... -
Pooling Samples for Sequencing
I am working on an experiment in which we are going to be doing reduced representation bisulfite sequencing and RNA-seq. Our plan is to pool the samples... -
Pooling samples for library prep using True seq
We have used barcoded primers for a PCR and our aim is to pool 8 samples together before subjecting to library prep using True seq (a modified protocol...
