Hi,
I was wondering if anyone has experience in demultiplexing BCL files from Illumina with two different barcoding systems in one go?...
34 results in 0.0031 seconds.
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demultiplex BCL data with two different barcoding systems
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i5/7 (P5/7) Nomeclature
Why is i5/7 (P5/7) named 5 and 7?
I understand i5/P5 are on 5' end, so perhaps it makes sense to name it 5, but what about 7? Or it must be some... -
Fusion primers
Hello,
has anyone used or knows how can I get adapter sequences and MID (multiplex identifier) tags, can I buy it or a I need to design... -
Can primers for PCR be duplicated?
Complete beginner question here, don't laugh: If I have some primers that have been synthesized, and I am close to running out of them, is there any way... -
Primer3 issue
Dear all
I'm new here and I have a problem with Primer3 program.
I'm using MISA and Primer3 in pipeline. After I got *.misa... -
primer-dimer or adaptor-dimer
Hi, all,
I just prepared couple of libraries with the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina. After run them on a Qiaxcel... -
KAPA index adapter compatibility
Hello,
Has anyone used the KAPA single-indexed adapter kit with a library prep kit other than one of KAPA's? I would like to test out their newer... -
Doubt for primer removal: illumina
Hi All,
I was browsing online bioinformatics forum and stumbled onto query about primer, adapters that put me in thought about the process/pipeline... -
Demultiplexing PacBio sequences
Hi all,
I have the following problem:
I have a dataset containing circurlarized and linear DNA. It was amplified with two pairs of primers... -
Primer3 primer3_core failed
Hi, I select 11 microsatellites generated by MISA program (http://pgrc.ipk-gatersleben.de/misa/), now I want to design primer, and I use this.
... -
Automated primer walking
Hi all,
I'm trying to generate a list of oligonucleotide primers for Sanger sequencing, such that for an arbitrarily large DNA sequence I would... -
SFF file - primers
Hello!
I am using 454 Roche Junior, I have few questions about reads of sff file.
I have splitted a SFF file into individual SFF file (according... -
how to check the primers specificity in a local computer
Could someone please tell me how to check the specificity of primers in a local computer? I know there are lots of online tools available, i.e. primer-blast.... -
using primer3+ design primers received a warning?
When I use primer3+ to do the primer design. It gives out a warning:
Checking for poly-A and poly-T regions >= 7 bp, and potential hairpins... -
Where does my primer anneal?
This seems like a dumb question, but I haven't been able to figure it out.
I sequenced hemoglobin genes using primers I found online/literature....
