Hi,
I've prepared ChIP-Seq libraries using NEBNext Multiplex Oligos for Illumina (Set 1, NEB #E7335) along with the NEBNext Ultra II DNA Library...
7 results in 0.0028 seconds.
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ChIP-Seq library prep; trailing, primer dimer help?
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Getting rid of primer dimer for RNA-Seq libraries
Hey all, I just found several of my RNA-Seq libraries have primer dimers, I was wondering what is the ratio of my final product that is now diluted in... -
TruSeq RNA PCR Cycle Number
Hello,
I'm seeing two size populations in my final RNAseq libraries, one at ~280 bp which is the expected library size and a larger one at...Last edited by little_henry; 04-26-2016, 11:37 AM. -
Ugly gels after switching to 384w plate from 96
Hello,
My lab use's custom primer sets for HLA genotyping using the Illumina MiSEQ. We have optimized our multiplex primer sets for initial amplification... -
96 well PCR clean up
Anyone found a 96 well clean up method that will remove 100 nt/bp and smaller from ~250 bp product? Most give 100 bp as the cutoff, but we've gotten... -
Strange Illumina library artifact.
We ran some "RAD" (Restriction site Associated DNA) tag libraries on our Illumina HiScanSQ. The libraries were not constructed by us, but the... -
Low Molecular Weight sample and primer dimers
I recently ran a Low Molecular Weight sample on our 454 and got a bad result. Most of the sequences were just primer dimers. Our samples are pooled...
