Hi,
Forgive me if this is naive, but I'm still newish to thinking about sequencing. I've begun working with the DADA2 software and the tutorial...
7 results in 0.0033 seconds.
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should primers be considered biological sequence?
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Forward and reverse reads overlap but don't align properly
I am working with Archaea F and R reads acquired with the 344f and 915r primers. Each read is 300bp long so there is overlap but it is only 29 bp. I want... -
ATAC-seq in mouse cell lines
Hello,
This is my first post. I have done multiple ATAC-seq libraries on human cell lines with no problems. Now I'm doing some ATAC on mouse lines.... -
ATAC-seq (Read1/Read2/Index Sequencing Primers?)
Hi,
We recently prepared samples for ATAC-seq using primers from the Buenrostro et al paper.
Ad1_noMX: AATGATACGGCGACCACCGAGATCTACACTCGTCGGCAGCGTCAGATGTG... -
Why A and B primers in SAGE? Why not just one?
Reading about SAGE (serial analysis of gene expression). Everywhere discusses adapters having A or B primer but no one explains explicitly why dividing... -
Nextera primers
Hi All,
In its customer sequence letter (5/20/2012) Illumina did reveal some primer sequences used in various kinds of application. But... -
Flow cell modifications ?
Hello,
Does anybody knows if there were some changes in the lengths and / or sequences of the primers attached to the Hiseq flow cells between...
