Really dumb question, but did Qiagen revive pyrosequencing when I wasn't looking?
https://www.qiagen.com/us/resources/technologies/pyr...
13 results in 0.0030 seconds.
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Qiagen "PyroSequencing"
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Analysis data with edgeR having some values missed
Hello everybody!
I'm trying to analyse some pyrosequencing data that some colleges sent me. I could get all experiment replicas for each... -
estimation of the expression changes
Hello everybody,
My colleges send me its pyrosequencing results to be analysed. the data that i got is a list of averages for different... -
Quantification Alternative
Hi guys,
I have recently been performing runs on the Roche GS Junior platform. I am using the platform for viral discovery from affected... -
pyrosequencing decreasing/sloping curve in negative control
Is anyone having similar problem and could give me answer why sometimes curve in negative controle is decreasing and sometimes is straight while checking... -
Need help to prepare sample for pyrosequencing
Hi
I am quite new to Pyrosequencing and therefore have some strange question!
I am going to analyse 60 gut samples using... -
454
I would like to sequence 16s from more than 400 sample of nasopharyngeal samples by pyrosequencing.
It seems to be a good method to do... -
Hello SEQanswers Community!
It feels great to have been introduced to this community by a dear friend.
I have high expectations about this great community of learners... -
Extract sequence from single OTU 454
Hi everyone,
I would like to extract a single sequence corresponding to an OTU obtained in the analysis of the 454 pyrosequence.
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The new flow pattern for FLX+
Has anyone updated their FLX+ machine to 2.8 software with the new flow pattern? Does it work? We are very reluctant, mainly because last time roche touched... -
454 Pyrosequencing error rate assesment tool
Hi,
Is anyone aware of some software or documented algorithm for estimating (separately) the error rate of PCR and sequencing for 454 data,... -
What does the FLX acronym mean?
Hello everybody,
I`d like to know what mean the FLX acronym in 454 sequencer?
Thanks -
Non-specific band after gel purification of PCR products
I'm trying to purify my PCR products (obtained by using barcoded fusion primes) by gel purification to increase the quality of amplicon pyrosequencing....
