Are there any 3rd party service provider (repair/PM/service contract) for the Thermo Fisher Quant Studio 7 Flex qPCR instruments?
TIA
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QuantStudio 7 Flex - 3rd Party Service Options?
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How are you liking your NextSeq 2000?
My team has reached the "serious considerations" stage of purchasing a NextSeq 2000, and I'm curious to hear how others are liking (or disliking?!)... -
Looking for a standardized method of library quantification and quality control
Hello all,
I am wondering if anyone out there has discovered/created a standardized method for library quantification and quality control... -
Library degrading after prep with Ovation Ultralow V2
Hello,
I am having an issue with library pools rapidly degrading after pooling and size selection.
I am prepping low biomass... -
Library degrading after prep with Ovation Ultralow V2
Hello,
I am new to this forum and NGS but the have found endless answers here to every issue I have run into. Hopefully you can help me... -
ragan1 started a topic I am trying to do a qPCR analysis of a miRNA in the rat brain that needs to control fin GeneralI am trying to do a qPCR analysis of a miRNA in the rat brain that needs to control f
I am trying to do a qPCR analysis of a miRNA in the rat brain that needs to control for treatment, sex, and siblings and I'm having trouble w/stats. Usually... -
Illumina library quantification using a QuantStudio 5
Hello,
Does anyone have any successful experience using a QuantStudio 5 to quantify their illumina libraries? I've been trying to get decent... -
qPCR for determining ATAC-seq amplification cycles?
Dear SeqAnswers,
From my understanding of the Buenrostro et al ATAC-seq papers, they PCR amplify transposed DNA fragments with 5 PCR cycles... -
ATAQ-Seq qPCR troubleshooting
Hello,
I am attempting to perform ATAQ-seq on human macrophages. I have performed the protocol, though have encountered an issue at the... -
Low Concentration Library
Hi,
I made libraries using Colon and Lung Cancer Research Panel and quantify them using qPCR. I was wondering why some of the libraries... -
ATAC-seq in mouse cell lines
Hello,
This is my first post. I have done multiple ATAC-seq libraries on human cell lines with no problems. Now I'm doing some ATAC on mouse lines.... -
qPCR issues
Hello, we use Kapa qPCR kits to quantify our libraries before sequencing. Recently, the NTC wells are giving low cq values which means there are contaminants... -
Inconsistency between 16s sequencing with Illumina and qPCR
Hi all, I would like to ask your opinion about inconsistent results regarding the abundance of certain bacteria between sequence data and qPCR using the... -
RNAseq independent validation by qPCR
Hi All,
I was wondering if anyone is aware of any RNAseq papers which validated using qPCR (or similar) on a new set of samples? i.e. samples A,B,C,D... -
qPCR vs Qubit, big difference
We have prepared a TruSeq DNA PCR-free library, and quantification with Qubit gives roughly 10 times higher concentrations than qPCR (range 4-50 times...
