Hi,
This is a 'piece of string' question in trying to identify the 'ideal' instrument. Is there a well curated review/ discussion anywhere of...
58 results in 0.0050 seconds.
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Seq Instrumentation/ Run costs
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Bubbles in R1 only (NextSeq500)
Hi seqanswers! We have a new problem with our NextSeq: showers of small bubbles that appear only in R1 and cause high error tiles on the lower surface....Last edited by Chris Coldren; 10-29-2018, 11:13 AM. -
Opinion on FastQC output for HiSeq 4000 PE sequencing run
Hello,
I recently had four 400bp insert plant DNA libraries sequenced (2x150bp) using one HiSeq 4000 lane.
I've attached...Last edited by quokka; 07-30-2018, 06:45 PM. Reason: remove identifying information from attachments -
Opinion on FastQC output for HiSeq 4000 PE sequencing run
Hello,
I recently had four 400bp insert plant DNA libraries sequenced (2x150bp) using one HiSeq 4000 lane.
I've attached... -
Opinion on FastQC output for HiSeq 4000 PE sequencing run
Hello,
I recently had four 400bp insert plant DNA libraries sequenced (2x150bp) using one HiSeq 4000 lane.
I've attached... -
Advice on getting good PacBio libraries
Hi everyone,
We are having issues getting good quality PacBio libraries from Bacteria and microalgae. Specifically, we got low yield (76... -
Experienced sequencing providers for RRBS-seq?
Hi,
can someone recommend me a reliable sequencing provider for RRBS-sequencing? Our current provider struggles with this kind of application,... -
Optimizing DNA Extraction from Dried Insects
Hi all,
I am attempting to get enough (and good enough) DNA extracted from dried adult butterflies to do sequence capture. I've been told I need... -
TruSeq Stranded Total RNA final sample storage
Dear Seq-ers
I was wonderding if somebody has any experience with, if any, sample quality loss after preparing your samples with the TruSeq... -
Oligo Synthesis Companies/Services
Hi everyone!
I've had one person tell me that IDT is the go-to company for oligo synthesis. Why is this?
I've gotten... -
Conceptual Poor Alignment Understanding
Hello.
I am working on some RNAseq and received a few poor reads from our sample set. One possible undersatnding is cDNA contamination. note we... -
Nextseq500 N's from position 25
Hi,
I've been seeing a lot of Nextseq500 runs lately and I found a very disturbing phenomenon. In a lot of the runs I start seeing a lot of N's... -
Influence of quality of genome assembly for RNA-seq read mapping
Dear all,
I am wondering how much the quality of the reference genome assembly influences RNA-seq read mapping (for example Illumina reads mapped... -
VCF file / relationship between QUAL and DP fields
Can someone explain to me if there is a relationship between QUAL en DP fields in a VCF file.
If I understand well, a high DP value (coverage)... -
illumina quality scores first 2-3 bp reliability?
Hi All-
I apologize if this has been asked- I searched and could not find any answer that address this question-
I was speaking to...Last edited by rufessor; 11-10-2014, 10:41 AM.
