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Quality trimming prior to aligning
I am aligning reads to the GrCh37 human reference genome using STAR and am confused as to how to get the most reliable results. At first I aligned my...Last edited by gstone; 02-28-2017, 03:48 PM.
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Struggling with quality trimming
Hello everybody,
this is my first try to trim Illumina (paired-end) reads on the unix command line.
If i get it correctly, de-multiplexing...
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How does BWA MEM handle reads shorter than 70bp?
Yet another quality trimming question... I've got some Illumina paired-end DNAseq data from tumor and normal samples (100bp reads). I want to align the...
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trimming or filtering first?
Does the order of trimming reads first and then filterings or first filtering reads and then trimming make a diffrence?
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bwa-mem and quality trimming
I want to switch from using bwa aln+sampe to bwa-mem, but I notice that there is no quality soft-trimming in the latter. (It is present in bwa aln). Am...
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Trimmomatic: invalid FASTQ name line
Hi all,
I'm trying to trim the adapters of my Illumina PE reads using Trimmomatic.
java -classpath trimmomatic-0.22/old/Trimmomatic-0.20/trimmomatic-0.20.jar...
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Adapter trimming and trimming by quality question
Hi to all,
I've been experiencing some problems trimming the adapters and filtering by quality my paired-end Illumina reads.
...