I want to switch from using bwa aln+sampe to bwa-mem, but I notice that there is no quality soft-trimming in the latter. (It is present in bwa aln). Am I missing something? Why has it not been included? Is there any other way to do this? Thanks!
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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