How does the Tapestation (2200/4200) measure fluorescently stained biomolecules ?
Does it scan fluorescence in real-time (during eletrophoresis)...
26 results in 0.0023 seconds.
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Tapestation imaging
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Looking for a standardized method of library quantification and quality control
Hello all,
I am wondering if anyone out there has discovered/created a standardized method for library quantification and quality control... -
Balance sequencing runs ATAC
I am having trouble balancing my sequencing runs for ATAC-seq. I am doing extensive kapa on the samples but often finding one out of a pool of 6 will... -
Illumina library quantification using a QuantStudio 5
Hello,
Does anyone have any successful experience using a QuantStudio 5 to quantify their illumina libraries? I've been trying to get decent... -
Salmon's strategy for aggregating transcripts into gene level
I am new in the field on RNA-Seq analysis and trying to understand the methodology behind Salmon's aggregation of transcripts into gene level.
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Question about library quantification prior sequencing
Hi There,
I have recently encountered a problem about library quantification that has hindered my project for months. And i have browsed... -
Quantification for transcirpt without reference genome.
Hello, every one!
I have got the transcript downloaded form NCBI. Also we have got the RNA-Seq data from illumina platform. Now we want quantify... -
how can I get abundance from both Iso-seq output and illumina data?
Hi,
We've got the done the RNA-seq of a species (genome is about 3GB), but NO reference genome is available, using pacbio and Illumina. After the... -
Quantitation Variations with Qubit
Hello All,
I have run into quite a strange issue recently.
I have successfully and consistently been running 16S and 18S... -
Memory requirments for eXpress
Does someone know how much memory eXpress usually consumes? (https://github.com/adarob/eXpress)
I am using eXpress together with kma (... -
Hello from a core facility for diagnostics and research
Dear users,
I have joined SEQanswers today and would like to introduce myself.
I have recently started working at a NGS... -
Quatification of tRNA-derived RNA fragments (tRFs) ?
Hi,
In the lab we are analyzing tRNA-derived RNA fragments. My boss needs to quantify these fragments in different conditions (Presymptomatic...Last edited by diego diaz; 01-19-2015, 05:04 AM. -
Salmon: A fast and versatile new tool for RNA-seq quantification
Hi all,
I'd like to let you all know about Salmon, a new tool we've been developing for isoform-level quantification from RNA-seq data.... -
Quantification Alternative
Hi guys,
I have recently been performing runs on the Roche GS Junior platform. I am using the platform for viral discovery from affected... -
Library quantification of Small-RNAseq
Hi!
We are doing Small-RNAseq libraries since a little bit (using TruSeq Small RNA prep kit) and we have problem quantifying the libraries accurately....
