Hi All,
I'm trying to get an idea of the processivity of Nanopore. For instance, do the pores reliably read the full length of 5 kb DNA...
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Nanopore: Input cDNA Length vs. Read Length?
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Illumina NextSeq shorter fragment size than expected
Hi,
We were preparing library for NextSeq whole exome sequencing. Our starting concentration before library preparation for three samples...
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different read lengths in rna-star
Hi,
I have a data set with several samples of length 76 and others of read length of 100 bases.
I would like to analyse...
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ion proton. Reads length much shorter than library size?
Hi,
Recently I'm doing RNAseq on ion proton. I used ion total-RNA seq v2 kit to make the libraries and checked the size distribution on...
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Fusion-gene finding (in search for THE read-length)
Hi all,
I am very new to fusion-gene discovery and I am tasked along with some fellow colleagues to apply bioinformatic methods on clinical...
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Count C versus read length
Hi,
I've had a good look through the bioinformatics thread but as far as I can see nobody has asked this question yet. I am using bisulphite...
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read length a problem when mapping with MAQ
Hi,
I have a file containing 36.8 million reads, as suggested in the MAQ manual I divided the file so that i had files containing 2 million...
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Illumina produced Read Length
I am comparing BowTie vs BWA for various read lengths. Now, before one year ago Illumina produced read lengths are typically 36, 75 or 100 bps which they...
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200-250bp read length
My application requires read lengths of 200-250bp, which looks doable with the 100bp and 150bp paired end reads on HiSeq and MiSeq. I was wondering...
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Comparing RNA-seq data with different read lengths
Hi All,
I work with SOLiD RNA-seq data. I have sequencing from the version 4 machine and sequencing from the 5500xl. These have different...
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Parameters of PaciBio
Hi,everyone! Does anyone know anything about the Pacific Bio seq instrument work stats? I knew the error rate is about 15% one year ago.
I wonder...
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ABySS contig coverage?
I've beebn playing around with ABySS and haven't been able to find much documentation on calculating contig coverage. The header to each contig reads...
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read length of GS FLX Titanium
So far, the average read length of 454 GS FLX Titanium system in our lab is 400 base pair, with most less than 400 base pair. Lately, a run got around...Last edited by anabio; 12-11-2009, 12:56 AM.
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BWA Read Length
Hello,
What is the maximum read length that can be used in BWA?
Thanks,
Anamika