Hi everybody,
I was approached by a biologist in our lab and was ask a specific question. As I was not really sure, I would like to share...
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library depths for sequencing
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Individual-level RNA-Seq differential expression analysis with paired sample design
Hi all, I'd appreciate any thoughts that will help us with the experimental design for our project.
Our experimental design involves collecting...
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Individual-level RNA-Seq differential expression analysis with paired sample design
Hi all, I'd appreciate any thoughts that will help us with the experimental design for our project.
Our experimental design involves collecting...
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RNA-seq bio-replication with low correlation
Hi!
I sequeced two biological replicates for one condition with Hiseq 2000 platfom.
but the typical R(Pearson) correlation of gene expression(Raw...
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Methylation Study
Hi,
I'm a PhD student and currently working on building up a Methylome of a plant species. I have a plant line which has been inbred for...
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Cuffdiff, replicates, and weird results
Hi everyone. Please let me know if this has been answered in another thread. I have a lab meeting coming up, so I'm a bit desperate to figure out this...
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Ref column for 2 factor RNA-Seq time course
Dear all,
I seek some help with normalization strategy for my experiment. I have described the experimental design below.
Tissue...