Hi!
I sequeced two biological replicates for one condition with Hiseq 2000 platfom.
but the typical R(Pearson) correlation of gene expression(Raw Count) between two biological replicates is only 0.93, ie the R2 only about 0.87.
Can I use these 2 samples to do the differencial analysis?
Any suggestion for how to use this to call DE genes?
Or some recommend readings are also very helpful.
Thanks all.
I sequeced two biological replicates for one condition with Hiseq 2000 platfom.
but the typical R(Pearson) correlation of gene expression(Raw Count) between two biological replicates is only 0.93, ie the R2 only about 0.87.
Can I use these 2 samples to do the differencial analysis?
Any suggestion for how to use this to call DE genes?
Or some recommend readings are also very helpful.
Thanks all.
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