9 results in 0.0026 seconds.
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Why would ligation fail with different isoschizomer?
We're having a weird issue with trying to ligate Illumina adapters to DNA extracted from cattle egrets (a bird species). Doesn't matter if the DNA is... -
Need feedback on RADseq using enzyme BbvCI
I am planning to genotype ~520 samples using original RADseq (Etter et al. 2011). The project has 2 goals: (1) construct a linkage map using a subset... -
PstI methylation sensitivity
So my lab is in the process of understanding and implementing a protocol for several species. One point of confusion we are trying to figure out is whether... -
Using Restriction Enzymes pre library prep for targeted sequencing.
Hey Everyone,
I'm using a targeted capture approach for sequencing the variable regions in Ig, we're trying to develop a basis for genotyping... -
biased mutation calling/false negatives from HALOPLEX datasets?
Hi everyone,
I'm wondering if someone has an opinion/experience with that. Using Haloplex, considering the frequent occurence of restriction... -
Cut Sites Per Megabase
Hey guys,
I can't seem to find the right tool to do this. I was wondering if anyone had advice on how to approach.
I am... -
help! RAD, Stacks process_radtags.pl script, ambiguous RADs
I produced a single digest RAD library using Sbf-I, a rare cutter, following a variation of the Etter (2011) protocol available on Michael Miller's website... -
in silico restriction digest of Illumina reads (fastq file)
Hi
I wonder whether anyone has a solution for a restriction digestion script that works on fastq files (Illumina reads), splitting any... -
bisulfite sequencing and cluster detection
Hi all, we are trying to perform BS-seq with the protocol described by Smith ZD et al - Methods 2009, which relies on preliminary digestion with MspI....
