Hi all!
I need help in selecting a restriction enzyme for GBS. Elshire's protocol for GBS calls for methylation-sensitive enzymes (They...
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Methylation-sensitive restriction enzymes for GBS
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Optimizing a ddRADSeq study with a large genome
I am designing a study to investigate the population genetic structure of an endangered species. We've decided to go the ddRADSeq route, and I have a... -
ddRADSeq Libraries
We seem to be having some difficulties creating reproducible ddRADSeq libraries.
We are noticing that after running the first of the processing... -
Rare cutter not cutting? (ddRAD)
Greetings, everyone!
I've been testing my restriction enzymes, and have candidates for a rare cutter and a common cutter. The rare cutter... -
Help on library, restriction enzyme fragmentation
Dear Everyone,
At our lab we are doing a sequencing project sequencing a short gene (1500 kb) in humans. The length of the amplicon does...
