Hi,
I have a fastq file with merged amplicon sequence reads post Illumina sequencing. However, the library was build by ligating the Illumina...
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Find_primer_sequence_and_reverse_complement_read_within_amplicon.fastq script needed
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d reverse complement sequences
Hi, I am new to RNAseq and not a bioinformatician so please take my apologies if these are basic questions. After mRNA Illumina PE sequencing of 6 brain... -
New Utility: Excel add-in for DNA/RNA string manipulations
I recently received some positive feedback for an Excel add-in that I pushed to github some time ago. that performs simple string manipulations (complement,... -
How can I distinguish assembly error from true splice or isoforms in RNA-seq?
Hii everyone!
I have received thousands of transcripts generated by a non-stranded RNA-seq and I have just annotated them (I used a in-house... -
Remove reverse complement redundancy in stranded transcriptome
Hi All,
We are working with a de novo transcriptome assembly of Illumina Hi-seq data - 20x 100 bp paired end, stranded libraries. Raw data underwent... -
Reverse complementary reads in Mummmer
The repeat-match program in mummer, claims to return reverse complementary repeats, and mark them with 'r'. However, I found that the repeats returned... -
Tophat strand-specific RNAseq- Do i need to reverse-complement?
Hi all
I have strand-specific RNAseq data ( using Truseq kit- dUTP method) and I aligned the reads onto the genome with Tophat using --library-type=fr-firststrand...
