Hi, everyone here!
Now I confuse about the exact position which reverse transcriptase stop at in SHAPE (Selective 2′-hydroxyl acylation analyzed...
3 results in 0.0030 seconds.
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Which nucleotide does the reverse transcriptase stop at in SHAPE reaction?
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Wouldn't an RNase+ reverse transcriptase give better library representation?
Hi All,
I will be building a library for mRNA Seq where I want to count transcripts (measure gene expression). Wouldn't using a reverse transcriptase... -
FRT-seq: amplification-free, strand-specific transcriptome sequencing
Brief Communication
Nature Methods 7, 130 - 132 (2010)
Published online: 17 January 2010 | doi:10.1038/nmeth.1417
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