Has anyone experienced seeing a prominent non-rRNA peak after rRNA-depletion? I am seeing this consistently in my samples.
See the attached...
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Prominent non-rRNA Bioanalyzer peak after rRNA depletion
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RNA on a DNA High senstivity chip
I am trying to determine if my DNAse I digest worked and I only have total RNA present in sample (for transcriptomic analysis). This sample was cleaned... -
RNA Degradation or DNA Contamination
Hi all,
I have been attempting to extract RNA (RNeasy Micro Kit) from mouse retinal cells collected using laser capture microscopy, but... -
How to define RNA quality for Drosophila RNA using Agilent Bioanalyzer?
Hi all,
I extracted RNA from Drosophila heads, and want to do RNAseq on them. I used Agilent's Bioanalyzer to assess RNA quality, and found... -
2100 Bioanalyzer, strange sharp peak in precursor region
Hi All!
I usually get a sharp peak in the precursor region, and I don’t know what can cause this anomaly!?
Details:
I extracted... -
sharp peak in library - TruSeq Stranded Total RNA (rRNA-depleted E. coli)
Anyone seen anything like the sharp peaks at 250 bp in the bioanalyzer traces of the libraries below? Overamplified? Bubble product?
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Bioanalyzer2100 RNA Pico Chips Expiry
Hello,
For the actual plastic chips (RNA 6000 Pico), they all have an expiry date. Is this real? In other words, has anyone used unexpired... -
Problem with the insert size of RNA-seq paired end reads!
The problem is that I cannot get a huge part of my RNA-seq data aligned!
These are some samples from the liver, intestine and colon of mice. We...Last edited by rozitaa; 02-24-2015, 05:21 AM. -
Strange RNA Bioanalyzer trace, any thoughts?
We have only just started using the Bioanalyzer RNA 6000 Nano chips, and I haven't seen a good trace yet. Today's was very strange....
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