Hello all,
I am extracting total RNA from tissues derived from wild animals after 24-48 h of death. I am able to get a RIN of approximately...
11 results in 0.0027 seconds.
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RNA purification, DNA contamination, and small RNA sizing profiles
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How to define RNA quality for Drosophila RNA using Agilent Bioanalyzer?
Hi all,
I extracted RNA from Drosophila heads, and want to do RNAseq on them. I used Agilent's Bioanalyzer to assess RNA quality, and found... -
RNAprotect Cell Reagent
So Qiagen has this RNAprotect Cell Reagent (https://www.qiagen.com/us/shop/lab-b...ringinformatio... -
RIN issues & Nanodrop/Bioanalyzer disparities
Hi All,
I am at my wits end and was hoping someone might provide some insight. I am working on a transcriptomic project with microfilariae (immature... -
Getting RIN score from Agilent's mRNA program?
Hello all (1st post here!),
I recently ran some RNA extraction samples on Agilent's Bioanalyzer for QC check. However, I realized afterward... -
Hi all, newbie LCM-RNAseq user here
I am interested in finding out the best methods to sequence RNA from laser capture micro dissected (LCM) mouse brain tissue. I will have low quantity... -
Help with determining RNA quality for RNA SEQ
Hi All,
I'm new to RNA-Seq, so I'm not that familier with RNA quality checks for RNA seq. I have 6 total bacterial RNA samples, that I... -
Total RNA stability following extraction/isolation
Hi all,
I will be extracting total RNA from animal tissue using the RNeasy minikit. The final step of which is to elute in to RNase-free... -
RNASeq from total RNA with a RIN under 8
Hi,
Has anyone got any experience in carrying out RNASeq from starting material with a RIN less than 8?
How well did it work?
We... -
Illumina Trueseq RNA prep kit problems
Hello all,
I have recently gone through the Illumina Trueseq RNA sample prep kit with no success. Has anyone else had problems with this... -
multiple rRNA peaks Biorad Experion
Dear All,
While testing my RNA samples for quality on the BioRad Experion i noticed multiple peaks before the 18S peak. The pattern is...
