Hi all,
I have been receiving some confusing output with an RNA-seq dataset that I am trying to ultimately determine differential expression...
17 results in 0.0023 seconds.
-
HiSAT2 RNAseq alignment low + stranded data predicted to be unstranded
-
RNA-Seq Library Prep in 2-Hours!?
BioDynami has launched the fastest RNA-Seq library prep kit in the market. With our technology, you can easily make high quality RNA-Seq libraries in... -
Two cDNA Peaks on Bioanalyzer
I used 200 ng total RNA input and the KAPA HyperPrep Kit to generate cDNA libraries. Usually, our libraries look like Sample 1 in the attached pdf, but... -
Single kit for bacterial RNAseq projects (from clinical samples)?
Hello,
Does anyone know of a standalone kit or protocol which could deplete mammalian rRNA and bacterial rRNA to be left with bacterial... -
Scriptseq complete gold epidem
I have total RNA extracted from environmental samples that I intend to sequence. The main aim is to detect viral and bacterial transcripts.
... -
Ransom Library prep for GC-rich genes
Hi,
We would like to do differential expression analysis, we did a genome (Illumina + PacBIo) and a transcriptome (Kapa mRNA kit) but GC-rich genes... -
adapter contamination in reads?????
hello everyone,
I would like to ask one qus: why am I getting 3'adapter sequences in R1 and R2 reads (as according to seq... -
How much DNA contamination "allowed"?
Hi,
We're going to do a RNA-SEQ experiment (with Nextseq 500) with bacterial RNA for differential gene expression. We have cells lysed... -
RNase III digestion time for Ion Total RNA-seq
We are doing poly A selection from 100ng of Total RNA. After building the libraries, the insert size looks to be around 100bp. We are wondering if the... -
SMART-seq2 and Nextera XT DNA sample preparation kit
Hi,
I was preparing 3 libraries for RNA-seq starting with 0.03, 0.3 and 3ng total RNA using the modified protocol for SMARTer ultra low... -
De novo Transcriptome and size selection
Hello,
I would like to have your opinion on this:
Would it help me for the my transcriptome assembly if I first made a size selection during... -
TruSeq PCR Prep Box - DNA/RNA
Hi all,
I wonder is there any difference between TruSeq DNA PCR Prep Box and TruSeq DNA PCR Prep Box?
Looks like we accidentally...Last edited by MLog; 10-28-2012, 03:39 AM. -
Adventures in cluster density titration.
We have perennial issues with obtaining sane/consistent library concentrations prior to clustering. Those of you with a HiSeq will probably have this... -
RNA-seq libraries - double peak after size selection
Hi everyone,
I get some weird results when measuring my RNA-seq libraries on Bioanalyzer and now desperately need an advice...
The libraries...Last edited by MLog; 02-08-2012, 02:46 PM. -
RNASeq from total RNA with a RIN under 8
Hi,
Has anyone got any experience in carrying out RNASeq from starting material with a RIN less than 8?
How well did it work?
We...
