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Larger Datasets Reveal Additional Switches That Control Gene Expression
Most GWAS loci fall in regions that control gene expression rather than in the genes themselves, and linking those variants to eQTL and sQTL signals has...
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RNAseq and Single Cell Analysis Online Courses @ Edinburgh Genomics
Do you have a project planned that involves RNAseq or single cell analysis? Or looking to gain skills in exciting new areas of genomics? Our upcoming...
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EMBL-EBI on-site course | Introduction to RNA-seq and functional interpretation
EMBL-EBI on-site course | Introduction to RNA-seq and functional interpretation | 20 – 24 February 2023.
Gain an introduction to the technology,...EMBL-EBI Train online course
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Illumina RNA-Seq Library Prep Update (Webinar)
Am 8. Juli um 14:00 Uhr findet ein Illumina Webinar zum Thema RNA Library Prep statt.
Es werden folgende Themen behandelt:
- Einführung...
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How to perform Pearson correlation coefficient calculations on RNA seq fold change va
I have gene expression data from different conditions from different studies. Instead of using the actual TPM values for Pearson Correlation coefficient...Last edited by Jayesh; 12-19-2019, 10:30 AM.
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10x Single-Cell RNA seq GEMS storage
I have a stock of 10x 3' v3 GEMS that was left at room temperature for 24 hours. 10x Genomics advises storage at room temperature no longer than 8 hours....
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DESeq2 multiple comparison
Dear Seqanswers community:
I ran into a question when I was doing RNA-seq analysis using DeSEQ2. I have 4 groups, 3 samples per group,...
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Introducing Skymap: a single data matrix for each omic layer for each species
Skymap is a standalone database that aims to offer:
1. a single data matrix for each omic layer for each species that spans >200k sequencing...
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Bioinformatics analyst at the University of Virginia
Visit http://jobs.virginia.edu/applicants/...uickFind=83906 to apply.
The Bioinformatics Core in the UVa School of Medicine seeks...
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Comparing MiSeq output from different runs
Hi, I have about 4 different MiSeq runs i have data for. I have pipelined the fastq files through bowtie-samtools-cuffdiff to compare cDNA libraries of...
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low GC% peak in one end of paired end reads
Hi,
I have paired end RNA seq data prepared from Brassica napus using TruSeq kit. After adapter trimming, FastQC shows a second low GC% peak per...
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Multi species RNA-seq comparison
Hi all,
I have count tables from two species. I'd like to find the find orthologous genes between the species and compare how the orthologs...
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Quantifying mRNA levels
Hello,
How do we quantify the mRNA level of a gene present in a tissue? I am looking for the absolute mRNA levels of certain genes present...
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Beginner question for Differential Expression Analysis
Hello,
I am a beginner in analyzing data from an RNA seq experiment. I was not the one performing the bioinformatics analysis (I am more...
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SNP calling from RNAseq data
Hello NGS fellows,
I am a newbie here and would highly appreciate your advice about one particular experimental design.
...