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RNAseq and Single Cell Analysis Online Courses @ Edinburgh Genomics
Do you have a project planned that involves RNAseq or single cell analysis? Or looking to gain skills in exciting new areas of genomics? Our upcoming...
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EMBL-EBI on-site course | Introduction to RNA-seq and functional interpretation
EMBL-EBI on-site course | Introduction to RNA-seq and functional interpretation | 20 – 24 February 2023.
Gain an introduction to the technology,...EMBL-EBI Train online course
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Illumina RNA-Seq Library Prep Update (Webinar)
Am 8. Juli um 14:00 Uhr findet ein Illumina Webinar zum Thema RNA Library Prep statt.
Es werden folgende Themen behandelt:
- Einführung...
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How to perform Pearson correlation coefficient calculations on RNA seq fold change va
I have gene expression data from different conditions from different studies. Instead of using the actual TPM values for Pearson Correlation coefficient...Last edited by Jayesh; 12-19-2019, 10:30 AM.
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10x Single-Cell RNA seq GEMS storage
I have a stock of 10x 3' v3 GEMS that was left at room temperature for 24 hours. 10x Genomics advises storage at room temperature no longer than 8 hours....
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DESeq2 multiple comparison
Dear Seqanswers community:
I ran into a question when I was doing RNA-seq analysis using DeSEQ2. I have 4 groups, 3 samples per group,...
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Introducing Skymap: a single data matrix for each omic layer for each species
Skymap is a standalone database that aims to offer:
1. a single data matrix for each omic layer for each species that spans >200k sequencing...
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Bioinformatics analyst at the University of Virginia
Visit http://jobs.virginia.edu/applicants/...uickFind=83906 to apply.
The Bioinformatics Core in the UVa School of Medicine seeks...
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Comparing MiSeq output from different runs
Hi, I have about 4 different MiSeq runs i have data for. I have pipelined the fastq files through bowtie-samtools-cuffdiff to compare cDNA libraries of...
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low GC% peak in one end of paired end reads
Hi,
I have paired end RNA seq data prepared from Brassica napus using TruSeq kit. After adapter trimming, FastQC shows a second low GC% peak per...
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Multi species RNA-seq comparison
Hi all,
I have count tables from two species. I'd like to find the find orthologous genes between the species and compare how the orthologs...
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Quantifying mRNA levels
Hello,
How do we quantify the mRNA level of a gene present in a tissue? I am looking for the absolute mRNA levels of certain genes present...
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Beginner question for Differential Expression Analysis
Hello,
I am a beginner in analyzing data from an RNA seq experiment. I was not the one performing the bioinformatics analysis (I am more...
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SNP calling from RNAseq data
Hello NGS fellows,
I am a newbie here and would highly appreciate your advice about one particular experimental design.
...
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RNA Seq analysis
I want to extract differential expressed genes using LIMMA from RNA seq data for three cancer types viz breast, lung and prostate. These data should have...