Hi all, I'd appreciate any thoughts that will help us with the experimental design for our project.
Our experimental design involves collecting...
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Individual-level RNA-Seq differential expression analysis with paired sample design
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DESeq2 workflow problem
Hi everyone,
I'm investigating differentially expressed genes in my wild-type yeast vs a mutant strain. Each strain has been cultured... -
Preparing reference transcriptome: to or not to megablast
A quick question here. I am constructing a reference transcriptome for DE out of 16 transcriptomes (8 control + 8 treatment) and my strategy is as follows:... -
DGE among two fairly similar conditions
I have illumina RNASeq data for of 3 samples each with untreated and treated conditions. Thus i have three biological replicates for each conditions....
