I have illumina RNASeq data for of 3 samples each with untreated and treated conditions. Thus i have three biological replicates for each conditions. I am not expecting much change after the 'treatment'; What parameters of DESeq and Cuffdiff do I need to tweak to handle conditions with more 'similarity' than 'difference' ? what normalization method should be used in cuffdiff2 , the default geometric mean normalisation, upper quartile normalisation (-N) or --raw-mapped-norm ?
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...