I have illumina RNASeq data for of 3 samples each with untreated and treated conditions. Thus i have three biological replicates for each conditions. I am not expecting much change after the 'treatment'; What parameters of DESeq and Cuffdiff do I need to tweak to handle conditions with more 'similarity' than 'difference' ? what normalization method should be used in cuffdiff2 , the default geometric mean normalisation, upper quartile normalisation (-N) or --raw-mapped-norm ?
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Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM