Hello,
I am implementing my pipeline for Roche 454 Junior and I have used GATK for called of variants.
I have compared my variants with Roche's...
11 results in 0.0022 seconds.
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homopolymer, steps after alignment
Hello.
I am implementing my pipeline and I have to start the steps after alignment. I have see on GATK pipeline that I have to do local realignment... -
problems with emPCR for GS Junior
Hi All,
I have an unusual problem with the consistency of emPCR kits that were received two months ago (emPCR oil and breaking kit (Lot:... -
The new flow pattern for FLX+
Has anyone updated their FLX+ machine to 2.8 software with the new flow pattern? Does it work? We are very reluctant, mainly because last time roche touched... -
hello and first questions!
Hello there!
I'm starting with my brand new data from Roche's 454 pyrosequencing and I have some questions at this point.
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acknowledging the seqanswers.com forum
Dear All,
Let me introduce myself as a researcher in biology who uses genomics approaches to solve biological problems and address... -
[Help] - 454 data analyser
I'm currently in 2º semester of my 1º year of master degree and we have now to do project. I choose to analyse data from DNA sequencing from Quercus... -
Pico Green vs Real Time PCR
Does anyone have any experience with quantifying 454 libraries using qPCR (Real Time) instead of Pico Green. Our recent attempts have shown at least a... -
covaris shearing settings for paired end
Hello,
Does anyone use the Covaris to shear their samples in the Roche 3kb paired end library preparation step of circularised DNA fragmentation?... -
SFF Read names
Is there anyone here who knows how the read names are assigned to reads in the SFF-output from a 454 sequencing round. I have multiple reads with the... -
"beginner in alignment" question
Hello,
I am analyzing Roche 454 sequence data. The sequencing was performed not for whole genome but for the exons of (around) 100 genes. When...
