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WGBS vs RRBS
DNA methylation is an epigenetic modification that occurs most commonly at cytosine nucleotides. A methyl group binds to the fifth carbon of the cytosine... -
In the Lab: A Closer Look at DNA Methylation Sequencing Techniques
1. DNA Methylation: An Insights into the Epigenetic World
In the ever-evolving realm of molecular biology, the study of epigenetics has paved...Last edited by Novogene; 08-29-2024, 07:07 AM. -
Methratio file
Hello everybody!
I would like to ask for some help with files obtained with methratio script from bsmap.
I'm doing a RRBS... -
Strand information in DMR identification and annotation
Hello,
Does anyone have any information/advice on whether strand information from aligned reads is taken into account during the identification... -
SNP calling from RRBS data
Hello,
I am interested in calling SNPs from reduced-representation bisulfite sequencing (RRBS) read data. The purpose of this analysis... -
Background for GO term enrichment analysis of RRBS DMR data?
Hi,
I am attempting to carry out GO term enrichment analysis on set of differentially methylated genomic regions (DMRs). The program I am... -
RRBS mapping efficiency in CLC vs Bismark
Hi
we are working with RRBS data from bull sperm samples. previously we used Bismark mapper for pig data and the results were very good... -
trimming paired end reads using Trim galore
Dear all
im trying to trim paired-end RRBS illumina reads using Trim galore. as far as i understood, paired end reads must be trimmed... -
comparing sequencing runs across Illumina machines
Hello SEQanswers community,
I am preparing to conduct a RRBS study of a conifer (with an estimated ~30Gb genome), and I would like to first... -
BiSeq - How to handle covariates?
Hi,
I am attempting to use BiSeq (Hebestreit et al. 2013) to delineate DMRs among treatment groups in Bismark aligned RRBS data
... -
Some Covered CpG sites is extremely high for RRBS with BiSeq package
I am using BiSeq package following bismark tools for RRBS analysis. I termed Covfiles as a BSraw file to include all the used 20 samples, and below is... -
Advice on DNA Extraction from RNAlater stored tissue
Hi,
I am attempting to extract DNA from RNAlater stored tissue samples for
downstream DNA methylation sequencing (RRBS).
... -
Experienced sequencing providers for RRBS-seq?
Hi,
can someone recommend me a reliable sequencing provider for RRBS-sequencing? Our current provider struggles with this kind of application,... -
Low DNA yield after adapter ligation
Hi guys,
I am generating RRBS library recently. In the whole process I use Beckman Ampure Xp beads to purify (cleanup and elute) DNA. But... -
Multiplexing RRBS samples
Dear everyone,
Im preparing a RRBS library. I want to multiplex 10 samples from 150ng DNA.
We use ilumnia DNA nano LT adaptors...
