Hello everyone!
I recently prepared a RNA library (RNA extracted from cultured fibroblasts) and ran it on a NextSeq 500, using the Illumina...
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rRNA contamination and streptavidin beads
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high rRNA after poly(A) selection
Hi,
About half our samples post library prep (poly(A) selection) + sequencing had around 50% rRNA content. The samples were re-prepped... -
Removing overrepresented sequences (rRNA contaimation) from RNAseq raw data
Hello,
I recently got data back from a single-end RNAseq run. After looking at the fastQC report, there were many overrepresented sequences... -
rRNA and adapter removal bondaries in RNA-seq HiSeq 3000 Illumina
Hello
I am new in RNA-seq analysis and it is difficult for me to find out info about quality assessment in the matter. I did an analysis... -
yeast rRNA contamination: Illumina prep
Our lab is making a library from yeast total RNA. We have tried NuGen kit, and got huge rRNA peaks in the samples. We then tried Illumina's RNA-Seq...
