Hello everyone!
I recently prepared a RNA library (RNA extracted from cultured fibroblasts) and ran it on a NextSeq 500, using the Illumina...
6 results in 0.0099 seconds.
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rRNA contamination and streptavidin beads
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Prominent non-rRNA Bioanalyzer peak after rRNA depletion
Has anyone experienced seeing a prominent non-rRNA peak after rRNA-depletion? I am seeing this consistently in my samples.
See the attached... -
Combining rRNA depletion with NuGen Ovation kit; no improvement in target enrichment
(first question on the forum
)
I am looking for some ideas and brainstorming on RNA-seq sample prep and data analysis.
I am working... -
Cannot find 18s and 28s ribosomal RNAs in the GTF file from UCSC
Hello everyone,
I couldn't find 18s and 28s rRNA's in the GTF file from UCSC genome browser. I want to map against hg19/GRCh37 genome.... -
MICROBEnrich 28S removal
I'm using Life Technologies' MICROBEnrich and MICROBExpress kits in conjunction to deplete prokaryotic/eukaryotic rRNAs and polyadenylated transcripts... -
Ribosomal depletion for RNA seq
We are currently embarking on an RNAseq project using a sequencing service provider but have a few questions concerning the ribosomal RNA depletion step...
