Hello,
Human RNA-seq data, after remove adaptor and primer, do I need to remove rRNA?
Thanks in advance for great help!...
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rRNA and adapter removal bondaries in RNA-seq HiSeq 3000 Illumina
Hello
I am new in RNA-seq analysis and it is difficult for me to find out info about quality assessment in the matter. I did an analysis... -
rRNA derived cDNA removal after library generation
I have a specific application that requires ~5ug total fragmented RNA input for an RNA-IP step, then library generation and removal of the rRNA derived... -
RiboZero kits
Hello,
After looking on the web, I haven't been really successful in finding information about the difference among the several rRNA removal... -
Ransom Library prep for GC-rich genes
Hi,
We would like to do differential expression analysis, we did a genome (Illumina + PacBIo) and a transcriptome (Kapa mRNA kit) but GC-rich genes... -
Filter out rRNA in variousspecies
Dear great helpers,
I'm new in RNA-seq analysis and I am confused with the idea about filtering the rRNA contaminated sequences. Would... -
Hi all, newbie LCM-RNAseq user here
I am interested in finding out the best methods to sequence RNA from laser capture micro dissected (LCM) mouse brain tissue. I will have low quantity... -
ribosomal RNA depletion
Hello,
I'm using Epicentre Ribo-Zero kit for removal of rRNA from Drosophila. I checked RNA on Agilent, before and after depletion, and I observed... -
High %rRNA in NuGen Encore invert libraries
We recently did a number of library generations using the Nugen Encore kit. The samples were from flies, Drosophila melanogaster; extractions and library... -
C.elegans rRNA reduction kits
I'm wondering if anyone out there has tried/compared multiple rRNA subtraction methods of C. elegans total RNA or has any comments on the matter?
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Bacterial RNA sequencing workflow for the PGM
Attached is a workflow describing how we isolated, ribo-depleted, and sequenced RNA from [I]E. coli[I] on the Ion Torrent PGM.... -
Globin & rRNA removal from blood RNA
The new Globin-Zero™ Gold Kit from Epicentre effectively removes the highly abundant adult globin mRNAs and the cytoplasmic and mitochondrial rRNAs from... -
New Ribo-Zero Gold Kit (Human/Mouse/Rat)
The Ribo-Zero™ Gold Kit (Human/Mouse/Rat) is an enhanced version of Epicentre's popular Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat). The Ribo-Zero... -
New Ribo-Zero rRNA removal kits for plant RNA
Epicentre's latest Ribo-Zero™ rRNA Removal Kits use an improved hybridization-capture technology that removes virtually all the cytoplasmic (nuclear-encoded)... -
New rRNA removal kit for RNA-Seq
EPICENTRE’s new Ribo-Zero™ rRNA Removal Kit (Human/Mouse/Rat) removes >99% of the 28S, 18S, and 5.8S and >95% of the 5S rRNA from both intact...
