9 results in 0.0040 seconds.
-
mitochondria in single nuclear RNAseq
for snRNAseq, do people use mitochondrial filtering? I'm not sure if this still makes sense even though we are working with data from nuclei rather than... -
Using scRNA-seq to help with snRNA-seq clustering
Hi,
I am working with a single nucleotide RNA-seq (snRNAseq) dataset. These are often hard to cluster compared to single cell RNA-seq (scRNAseq).... -
issues with single nuclei sequencing
Hi All,
I am working on a set of single-nuclei sequencing samples. Does someone know why I might be getting a bimodal distribution in one... -
10x genomics 3'RNA-seq dual indexing
Hello,
so I had a discussion with the 10x Genomics support people about sequencing the v3 chemistry 3'RNAseq libraries on a HiSeq3000/4000... -
10x Genomics Single-cell Half Reactions
Has anyone attempted or successfully done half reactions for 10x Genomics platforms?
I know that the Gene Expression kits can capture 500-10,000... -
Suggestions for thermal cyclers that work with Pico PCR plates
Dear all,
I am about to start a new set of single-cell RNA sequencing experiments, and I am trying to get the basics right. I have a very rare... -
Bacterial scRNA-seq experience?
Hi all,
I'm trying to perform a scRNA-like library prep in bacteria, so I cannot take advantage of the polyT reverse transcription primers.... -
Single Cell FACS before Sequencing
Hi all,
we are designing an experiment to compare a control vs. condition (diet based) in rats to trial the new BioRad/Illumina single... -
how to do differential expression gene analysis for single cell RNAseq
hi,
how to do differential expression gene analysis for single cell RNAseq?
I tried DESeq2 and Edger, this two method didn't considered...
