42 results in 0.0031 seconds.
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Circular RNA Sequencing
Hello Everyone, we aim to study Circular RNA in a few days. Can you give advice for optimization and Library Prep phase? I will be using the Illumina...Last edited by MehmetZeki; 01-26-2024, 08:24 AM. -
detect CRISPR edit using HRM
Hello,
I am trying to do knock-out in cell cultures using CRISPR.
The cells are being transfected with a plasmid containing the essentials... -
Count error rate of sam file
I have aligned the fastq file to the reference genome and got a sam file. I know use "samtools stats" can get "error rate" of the... -
Removing redundancy from GenBank plasmid database using cd-hit-est
Hey all,
I have the following problem. I have a plasmid sequence database (ftp://ftp.ncbi.nlm.nih.gov/genomes/refseq/plasmid/) that is... -
mouse or non-human cancer sequence download
Hi everyone,
I am asking for help,I need mouse or non -human cancer sequences to verify my SV detection tools,but I do not know where to download... -
Plasmid Database / API which offers data in JSON format
Hi everyone,
does anyone know about a decent Plasmid Database or an API that offers plasmid sequence and meta data in .json format? I just... -
Truseq Custom Amplicon kit overhang sequences
I have been searching for for the overhang sequence on the i7 and i5 indices of the Truseq Custom amplicon Index kit, but couldn't find them..
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Mafft mismatch value
Hi,
I tried to find both in
http://mafft.cbrc.jp/alignment/softw...al/manual.html
and
http://mafft.cbrc.jp... -
Sequence read (id) specific kmer frequency calculation
Hi,
How to count sequence read specific kmers from a single fasta file? I am working on Ulcerative Colitis Human Gut Microbiome data, where... -
what is third column in the log file output for pacBioToCa
I used pacBioToCa to correct some long reads LR using illumina short reads SR
from the log file there is this info:
... -
Comparing Merged Seqs to a Reference Seq
Hello,
I recently received paired-end reads from an amplicon library I sequenced with Illumina. I used BBMerge to merge the paired-ends... -
Bowtie2 and MapQ Calculation
Hi
I'm going to ask about bowtie2's mapping quality calculation for ChIP-Seq data. For sequences which align multiple locations Bowtie2...Last edited by KyuzoSeq; 03-08-2015, 05:47 AM. -
Tools to determine sequence contamination in Methyl-Seq data ?
Hi,
I have a question regarding determining sequence contamination from Methyl-Seq experiments.
As we all know, for Methyl-Seq... -
how to measure the quality of PacBio data
Hi,
How to measure the quality of PacBio reads? if you have multi cells What is the tools and How to measure the quality of the read to be...Last edited by Medhat; 08-30-2014, 02:19 AM. -
Parsing position in a protein sequence using perl
Hi!
I'd like to ask for help because I'm trying to deduce the relative positions in an ungapped sequence given the positions in a gapped...Last edited by dovah; 07-31-2014, 05:12 AM.
