Hello Everyone, we aim to study Circular RNA in a few days. Can you give advice for optimization and Library Prep phase? I will be using the Illumina Total RNA Ribo Zero Plus kit. My understanding is that to obtain circular RNA, I will need to add an extra cleanup and amplification step after the rRNA Depletion steps before creating First Strand DNA. Which enzyme should I use to obtain Circular RNA at that stage? Which one would you recommend? What exactly will the enzyme we use do? Does it have anything to do with the A-tailing stage?
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Last edited by MehmetZeki; 01-26-2024, 08:24 AM.
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