Hello Everyone, we aim to study Circular RNA in a few days. Can you give advice for optimization and Library Prep phase? I will be using the Illumina Total RNA Ribo Zero Plus kit. My understanding is that to obtain circular RNA, I will need to add an extra cleanup and amplification step after the rRNA Depletion steps before creating First Strand DNA. Which enzyme should I use to obtain Circular RNA at that stage? Which one would you recommend? What exactly will the enzyme we use do? Does it have anything to do with the A-tailing stage?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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