Hello!
I am using 454 Roche Junior, I have few questions about reads of sff file.
I have splitted a SFF file into individual SFF file (according...
11 results in 0.0023 seconds.
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SFF file - primers
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From where to download sfffile utility
Hi Members,
I'm newbie with SFF files, and their split, extraction, MIDs.
I'd like to get sff files for specific bar codes. I... -
bioinformatics
Hey guys!
I was wondering how should I interpret the quality scores coming from results from 454 GS jr cDNA (not paired end) reads?... -
454 fasta qual description format NGS QC toolkit Quality Trimming
Hello, I am using NGS QC toolkit to do a quality trimming. Specifically I am using the 454QC_PE.pl tool for 454 paired-end reads. It works perfectly... -
Extract paired end reads from sff file.
Hi,
I'm working on a genome assembly project from 454 paired end reads which are in sff format. I have already tried Newbler which works... -
extracted sequences from sff
Hi,
Has anyone successfully extracted a subset of sequences by their read ID from an sff file? I'm trying to separate plasmid and chromosomal... -
running Newbler with a lot off .sff files
I have around 1,00,000 .sff files that i want to use for doing an assembly with Newbler.
What would be the best strategy to use?
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SFF Read names
Is there anyone here who knows how the read names are assigned to reads in the SFF-output from a 454 sequencing round. I have multiple reads with the... -
fasta+qual = sff??
Dear Bioinformatists,
The data I have received has 454 pyrosequence reads in Fasta format and respective quality scores as a .qual file.... -
sff file size limit in Newbler?
I have tried using runAssembly on the sff files I received from the sequencing guys, but for one file I get an error message:
$ runAssembly... -
using sff_extract
being a newbie, I have a very simple question:
I am trying to convert sff formatted 454 data into fasta, fasta quality files and an xml...
