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Recommendation 150nt single-end vs 2x75nt paired-end
We are in the planning phase for an experiment that requires sequencing of a DNA pool of length about 140bp. It is crucial that the entire fragment is...
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Strand Rule Problem via rseqc in single end reads
Built an rna-seq pipeline for paired end reads.
Got single end data and had to revise.
Only problem I have now is the rseqc...
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why rG and not dG in the template switch oligos (TSO) in single cell library preps?
Hi all,
I am preparing single cell libraries for RNAseq using the SMARTer II kit (Clontech) and I was wondering whether there is a reason why they...