Hello,
We plan an RNAseq analysis to study gene expression in a bacteria that we have an in house reference genome assembly (~7.8Mbp) and gene...
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SE vs. PE for bacterial RNAseq
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overlapping paired-end reads vs single end reads
Dear all,
I am doing 2x100 paired-end (PE) exome sequencing on an Illumina machine with the Nextera rapid capture exome capture protocol....
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library type for single end RNA-seq data
I searched lot of threads but didn't find any concrete answer.
I have single end RNA-seq data generated by on a Hi-Seq 2000 and I am not...Last edited by imsharmanitin; 06-30-2015, 10:15 AM.
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How to use unpaired reads from trimmomatic output in SOAPdenovo for assembly?
Hi all,
I have illumina paired end reads trimmed using Trimmomatic - resulting in 4 files - 2 paired, 2 unpaired (as expected). Now I want...
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Mapping multiple FASTQ data types using BWA?
Greetings!
I plan to do mapping using multiple types of Illumina FASTQ files for the same sample.
I have one set of FASTQs...
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stampy error: Mixed single- and paired-end input
Dear forum members,
I am mapping a couple of samples using stampy with bwa mapping. Most of the samples map fine, but in two cases I get...