43 results in 0.0035 seconds.
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UEA sRNA Workbench error code
I'm trying to run a pre-configured workflow (quality check & differential expression) in UEA sRNA Workbench, however, I get an error code a few seconds... -
RNA purification, DNA contamination, and small RNA sizing profiles
Hello all,
I am extracting total RNA from tissues derived from wild animals after 24-48 h of death. I am able to get a RIN of approximately... -
Bowtie-build - reference genome issue
Hello!
I`m new in NGS analysis, but in my new lab i had to replace my colleague in big NGS project related to miRNA-seq. He left me bunch... -
Small RNA-seq with UDIs for Illumina® NovaSeq®, MiSeq®, & HiSeq® 2000/2500 sequencers
PerkinElmer’s NEXTFLEX® Small RNA-seq v3 kit is now available with UDIs for dual indexed miRNA sequencing on the Illumina® NovaSeq®, MiSeq®, and HiSeq®... -
GFF3 files for small RNA
Hi everyone,
I'm looking for gtf/gff/gff3 files for small RNA (sequencing run based on a MiSeq with small RNA kit). Does anyone know where to get... -
is it possible to filter small RNA from a regular transcriptome?
Dear all,
I was wondering if anyone has ever tried filtering (potential) small RNA from a regular RNAseq transcriptome .
I have 38... -
De novo small RNA assembly
Hello,
I want to de novo assembly small RNA reads generated from Illumina sequencing (final lengths after clipping and trimming are 18-32nt)....Last edited by Juulluu21; 06-23-2016, 06:56 PM. -
Problem generating library for miRNAs from plasma
Hi all,
We've been having a hard time generating a library from plasma miRNA to use with Ion Proton. So far we've tried Life Tech's RNAseq... -
Spike-in sets for small RNA seq?
I'm thinking of making a commercial offering of the SRQC and ERDN spike-in sets for small RNASeq: http://nar.oxfordjournals.org/content/early/2015/04... -
filter for sequences shared among replicates
Hi all,
I have small RNA sequence data for 5 treatment groups with 4 replicates each. For each replicate, I want to filter the read lists... -
Rfam/infernal help running
Hi all,
I am trying to use rfam to search for sequence homologs to many small RNAs (read lists of about 50,000 sequences).
... -
Quatification of tRNA-derived RNA fragments (tRFs) ?
Hi,
In the lab we are analyzing tRNA-derived RNA fragments. My boss needs to quantify these fragments in different conditions (Presymptomatic...Last edited by diego diaz; 01-19-2015, 05:04 AM. -
Small RNA Library Sequencing on NextSeq
Does anyone have experience sequencing small RNA libraries on the NextSeq? We are consistently getting low cluster densities (140-150K/mm2) even with... -
Which software can produce this format file?
Hi, every one. I recently prepared a research paper about small RNA seq. One reviewer hope me to provide a result file with following format. Who can... -
Can small RNA library be sequenced paired end?
Hi all,
I made a library that is compatible with TruSeq Small RNA protocol. In other words, I can sequence it by Miseq using small RNA sequencing...
